Bottom up identification of lipids from DDA data
LipidHunter is an open source software for the high-throughput identification of lipids from DDA data acquired by LC-MS/MS and shotgun experiments. LipidHunter resembles a workflow of manual spectra annotation. Lipid identification is based on MS/MS data analysis in accordance with defined fragmentation rules for each lipid class. The software tool matches product and neutral loss signals obtained by CID/HCD to a user-defined white list of fatty acid residues and lipid class-specific fragments. LipidHunter provides information rich tabular and graphical reports allowing to trace back key identification steps and perform data quality control.
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