Lipidomics Methods and Protocols

Analytical methods were selected from special issues and review papers published by LIPID MAPS® Consortium members, maintaining some of the traditional and introducing new ones with the latest technology and instrumentation. These protocols are specific to each lipid category but can be also applied to several types of biological matrices.

Methods organized by lipid category

Fatty acyls

Fatty acids

Eicosanoids

Glycerolipids

Mono/di/triacylglycerols, Monoether diacylglycerols

Glycerophospholipids

PA,PC,PE,PS,PG,PI

Sphingolipids

Multiple sphingolipid classes

Sterol lipids

Sterols

Cholesterol esters

Prenol lipids

Ubiquinones, Dolichols

Multiple lipid categories

Glycerophospholipids, Sphingolipids

Lipidomics methods

Overview of Quantitative Lipid Analysis by Mass Spectrometry (png) (PDF) - Flow chart outlining the procedure for lipid analysis of various lipid classes, from extraction to laboratory analysis to bioinformatics.
Chemical Reviews 111 (2011), Special issue: Lipid Biochemistry Metabolism and Signaling Edited by H. Alex Brown and Lawrence J. Marnett.
BBA - Molecular and Cellular Biology of Lipids 1811 (2011), Special issue: Lipidomics and Imaging Mass Spectrometry Edited by Alfred H. Merrill and Robert C. Murphy.
Methods in Enzymology, Academic Press 432 (2007), LIPID MAPS chapters - (PDF). For the complete Methods in Enzymology volumes, including LIPID MAPS chapters in Volumes 432, 433, and 434, please see http://www.sciencedirect.com/science/bookseries/00766879. References to individual chapters from these volumes can also be found on our LIPID MAPS Publications: 2007 page.

Qualitative Analysis and Quantitative Assessment of Changes in Neutral Glycerol Lipid Molecular Species Within Cells
Glycerophospholipid Identification and Qualitation by Electrospray Ionization Mass Spectrometry
Detection and Quantitation of Eicosanoids via High Performance Liquid Chromotography-Electrospray Ionization-Mass Spectrometry
Structure-Specific, Quantitative Methods for Analysis of Sphingolipids by Liquid Chromotography-Tandem Mass Spectrometry: "Inside-Out" Sphingolipidomics
Analysis of Ubiquinones, Dolichols, and Dolichol Diphosphate-Oligosaccharides by Liquid Chromatography-Electrospray Ionization-Mass Spectrometry
Extraction and Analysis of Sterols in Biological Matrices by High Performance Liquid Chromatography Electrospray Ionization Mass Spectrometry
The Lipid Maps Initiative in Lipidomics
Bioinformatics for Lipidomics
Quantitation and Standardization of Lipid Internal Standards for Mass Spectrometry

Lipidomics workshop at EB2009 - (PDFs) The LIPID MAPS Consortium presented a workshop on lipidomics techniques at EB2009 under the auspices of the ASBMB. The slides from each of these presentations contain references to most of the methodoligies described .